Figure 4.

Characterization of leukocyte profiles in Apoe^−/− Mc1r^e/e mice. A, B, Quantification of total leukocytes (CD45⁺), lymphocytes (CD45⁺, CD11b⁻), neutrophils (CD45⁺, CD11b⁺, Ly6G⁺), and Ly6C^low and Ly6C^high monocytes (CD45⁺, CD11b⁺, CD115⁺, Ly6C^low/high) in the blood and spleen of Apoe^−/− and Apoe^−/− Mc1r^e/e mice. C, Percentage of dead monocytes and neutrophils in the spleen of chow-fed mice. D, E, Number of monocyte-derived dendritic cells (CD45⁺, CD11b⁺, CD11c⁺) and macrophages (CD45⁺, CD11b⁺, F4/80^high) in the spleen of chow-fed mice. F, Representative hematoxylin and eosin (H&E)–stained and CD11b-stained spleen sections from chow-fed Apoe^−/− and Apoe^−/− Mc1r^e/e mice. Dashed lines indicate the areas of white pulps. Scale bar, 200 μm. G, Enumeration of total leukocytes, lymphocytes, neutrophils, Ly6C^high monocytes, and macrophages (CD45⁺, CD11b⁺, F4/80^high) in the aortic lysates from Apoe^−/− and Apoe^−/− Mc1r^e/e mice. n=6 to 10 mice per group in each graph. Samples for chow and 4-wk high-fat diet (HFD) groups were derived from different sets of mice. *P<0.05 and **P<0.01 vs Apoe^−/− mice. Values are mean±SEM. Apoe indicates apolipoprotein; and MC1-R, melanocortin 1 receptor.