Figure 3.

BMS-986120 reduced thrombus formation at high shear but not at low shear. A, Representative image of porcine aortic media exposed to human blood at high shear stained to quantify total thrombus area. Sections were stained with polyclonal goat antihuman fibrin(ogen) antibody and CD61 monoclonal mouse antihuman antibody before treatment with 3,3′-diaminobenzidine substrate chromogen. Sections were then counterstained with a modified Masson trichrome (hematoxylin and sirius red, 0.1%). Effect of (B) BMS-986120 and (C) aspirin (ASA)±clopidogrel (Clop.) on total thrombus area at high and low shear. Statistical comparisons (least significance difference test) vs 0 h are represented above each plot. ns indicates nonsignificant. *P<0.05, **P<0.01, ***P<0.001.