REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-GFP (Chicken)	Abcam	ab13970; RRID: AB_300798
Anti-GFP (Mouse)	Memorial Sloan Kettering Monoclonal Antibody Facility	Htz-GFP-19C8
Anti-AstA (Mouse)	Developmental Studies Hybridoma Bank (University of Iowa)	5F10; RRID: AB_528076
Goat anti-chicken, Alexa Fluor 488	Thermo Fisher Scientific	A11039; RRID: AB_2534096
Goat anti-mouse, Alexa Fluor 546	Thermo Fisher Scientific	A11003; RRID: AB_2534071
Streptavidin, Alexa Fluor 568	Thermo Fisher Scientific	S11226; RRID: AB_2315774
Chemicals, Peptides, and Recombinant Proteins
RNaseOUT Recombinant Ribonuclease Inhibitor	Invitrogen (Thermo Fisher Scientific)	10777019
cOmplete Protease Inhibitor Cocktail	Roche	11873580001
IGEPAL CA-630	Sigma	I3021
1,2-dihexanoyl-sn-glycero-3-phosphocholine	Avanti Polar Lipids	850305
Protein G Mag Sepharose beads	GE Healthcare	28944008
PicoPURE RNA Isolation Kit	Invitrogen (Thermo Fisher Scientific)	KIT0204
RNA 6000 Pico Kit	Agilent	5067-1513
SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing	Clontech	634890
Agencourt AMPure XP system	Beckman Coulter	A63881
LightCycler 480 Probes Master	Roche	04707494001
PFA	Electron Microscopy	15700
Normal goat serum	Sigma	G9023
AstA (SRPYSFGL-NH2)	PeptideSynthetics	N/A
Scrambled AstA (GRFSSYLP-NH2)	PeptideSynthetics	N/A
Tetrodotoxin citrate	Tocris	1069
All-trans retinal	Sigma	R2500
Vectashield antifade mounting medium	Vector Laboratories	H-1000
Experimental Models: Organisms/Strains
Drosophila: w1118	Bloomington Drosophila Stock Center (BDSC)	RRID: BDSC_3605
Drosophila: Canton-S	BDSC	RRID: BDSC_64349
Drosophila: w1118;+;R23E10-GAL4	BDSC	RRID: BDSC_49032
Drosophila: w1118; UAS-DenMark, UAS-GFPDSyd-1	David Owald	N/A
Drosophila: w1118;+;AstAMB10261	BDSC	RRID: BDSC_29107
Drosophila: w1118; UAS-AstARNAi	Vienna Drosophila Resource Center (VDRC)	103215KK
Drosophila: w1118; UAS-MipRNAi	VDRC	106076KK
Drosophila: w1118; UAS-AstA	This paper	N/A
Drosophila: w1118; UAS-EGFP::mL10a	F. Rob Jackson	N/A
Drosophila: AstA-R1MB07922	BDSC	RRID: BDSC_25577
Drosophila: elav-GAL4[C155]	BDSC	RRID: BDSC_458
Drosophila: w1118; UAS-AstA-R1RNAi	VDRC	101395KK
Drosophila: w1118; +; R22H05-GAL4	BDSC	RRID: BDSC_49002
Drosophila: w1118; +; R22H10-GAL4	BDSC	RRID: BDSC_49005
Drosophila: w1118; +; R24B11-GAL4	BDSC	RRID: BDSC_49070
Drosophila: w1118; R23E10-LexA	BDSC	RRID: BDSC_52693
Drosophila: w1118; UAS-CD8::GFP	BDSC	RRID: BDSC_32186
Drosophila: w1118; +; lexAop-CD4::mCherry	Scott Waddell	N/A
Drosophila: w1118; lexAop-P2X2	This paper	N/A
Drosophila: w1118; UAS-Kir2.1::GFP	Evan Harrell and Gero Miesenböck	N/A
Drosophila: w1118; +; tubP-GAL80ts	BDSC	RRID: BDSC_7017
Drosophila: w1118; UAS-CsChrimson	BDSC	RRID: BDSC_55135
Drosophila: w1118; +; R78A01-GAL4	BDSC	RRID: BDSC_39985
Drosophila: w1118; R48H04-LexA	BDSC	RRID: BDSC_53609
Drosophila: w1118; +; R58H05-GAL4	BDSC	RRID: BDSC_39198
Drosophila: w1118; R24B11-LexA	BDSC	RRID: BDSC_53547
Software and Algorithms
Sleep analysis from Trikinetics activity counts	Paul Shaw	N/A
Software for optogenetic stimulation and movement detection	This paper	N/A