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. Author manuscript; available in PMC: 2018 Jan 23.
Published in final edited form as: J Biosci. 2015 Mar;40(1):91–111. doi: 10.1007/s12038-015-9508-6

Figure 5.

Figure 5

Genomic integration of Cfs involves DNA double-strand break repair. (A) In vitro demonstration of γ-H2AX foci at sites of Cfs integration. NIH3T3 cells (10×104) were treated for 6 h with Cfs (5 ng DNA) labelled in their protein with ATTO-TEC (green) and metaphase spreads were prepared. Immuno-fluorescence images were developed using antibody to γ-H2AX (red). Co-localization of green and red signals were clearly visible. (B) In vivo demonstration of γ-H2AX foci at the sites of Cfs integration. Mice were injected i.v. with Cfs (100 ng DNA) and sacrificed 24 h later. Sections of brain were processed for immuno-FISH using human-specific genomic probe (green) and antibody against γ-H2AX (red). Co-localization of green and red signals are clearly visible.