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In situ glycan editing of cell-surface glycans. Specific labeling of glycans terminated with galactose is achieved by in situ Sia editing with CMP-SiaNAl followed by reaction with an azide probe via CuAAC. Modulation of cell signaling is achieved by remodeling cell-surface glycans via in situ Sia editing with CMP-Sia or in situ Fuc editing with GDP-Fuc.
