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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Jan 2;57(4):967–971. doi: 10.1002/anie.201706535

Figure 3.

Figure 3

Cell-surface in situ glycan editing suppresses EGFR signaling. a) A549 cells were labeled with CMP-SiaNAl or CMP-Sia and ST3Gal-IV, then reacted with biotin-Az via CuAAC. EGFR pull-downed from cell lysate were resolved and probed with anti-biotin antibody (left); biotinylated proteins pull-downed from cell lysate were resolved and probed with anti-EGFR antibody (right). b) Starved A549 cells were treated with or without in situ Sia or Fuc editing and stimulated with EGF. Lysates were prepared and analyzed by Western blotting (n =3, *p <0.05). c) Wound-healing assay for the effects of EGF, in situ Sia editing and sialylation inhibitor on cells migration. A549 cells were treated and wounded, the summarized migration area were measured using ImageJ (n =6, **p <0.01).