Table 2. Main characteristics of sequenced plasmids.
ESCR gene | Other ARG | Contigs | Cumulative contig size (bp) | Replicon and pMLST | Addiction systems | |
---|---|---|---|---|---|---|
Plasmids from commensal strains | blaCTX-M-1: 13a |
tet(A): 8 sul2: 13 blaTEM1b: 1 dfr1a:1 dfra17: 3 aadA5:3 |
1–31 contigs (mean: 6.2) | Mean: 119 kbp | IncI1 99.30%: G57A: 13 ST3 100%: 13 |
ParE-RelB: 11 |
blaCMY-2: 2 | - | 2 or 6 contigs | Mean: 100 kbp | B/O/K/Z 98% or 97% | RelE-StbD: 1 | |
Plasmids from diseased broilers | blaCTX-M-1: 17 |
tet(A): 14 sul2: 16 blaTEM1b: 1 dfra17: 2 aadA5:2 |
1–27 (mean: 6) | Mean: 110 kbp | IncI1 99.30%: G57A: 13 ST3 100%: 12b |
ParE-RelB: 12 CcdB-CcdA / COG5302: 1 |
blaCMY-2: 1 | - | 12 | 203 kpb | FIA, 100% 384/388 FIB 99.56%, FIC/FII 95.59% F18:A6:B42 |
VagC/ SpoVT_AbrB/ MazE-VapC/ PIN |
anumber of plasmid
bthe entire ardA gene could not be found in pCOV25, but the four other alleles (pilL_2, repI1_2, sogS_1 and trbA_4) were those of the pMLST3