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. 2018 Jan 23;14(1):e1007129. doi: 10.1371/journal.pgen.1007129

Fig 4. mph1Δ reduces rDNA replication defects in Nse5-Smc6 degron cells.

Fig 4

A. FACS profile showing first cell cycle progression of Nse5-Smc6 double degron (MPH1) and Nse5-Smc6 double degron mph1Δ (mph1Δ) cells using the same strategy shown in Fig 2A. B. Southern blots for Chr XII and Chr III showing that mph1Δ improves Chr XII replication. Chr XII and Chr III signals in wells and in-gel were detected, and percentage of chromosome gel entry was calculated as described in Fig 3D. Standard deviations and P-values (t-test, *p<0.05, ** p<0.01) are derived from n = 3 trials for rDNA and n = 3 trials for ARS305. C. mph1Δ reduces rDNA replication defect in Nse5-Smc6 double degron cells. Chromosomes were digested with XhoI before PFGE and Southern blot as in Fig 3E. Percentage of rDNA gel entry was calculated in Fig 3E. Standard deviations and P-values (t-test, *p<0.05) are derived from n = 3 trials.