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. 2018 Jan 23;13(1):e0190924. doi: 10.1371/journal.pone.0190924

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© 2018 Knytl et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Metaphases of hybrid CCAHe1Fi female (3n = 156), (A) Counterstained by DAPI showed all 156 chromosomes with nine positive blocks; B&W. (B) 5S rDNA probe showing 27 loci; green, three of nine DAPI-positive blocks co-localized with 5S rDNA fragments. Three strong signals are indicated by arrows. (C) Chromosomes stained by CMA₃ showing four NORs; B&W, (D) Pseudo-coloured with DAPI (blue), CMA₃ (green) indicated by arrows. (E) C-banded chromosomes showing 27 heterochromatic blocks situated in the pericentromeric chromosome regions; B&W, (F) Pseudo-coloured with DAPI (blue). Scale bars = 10 μm.