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. 2018 Jan 23;13(1):e0190924. doi: 10.1371/journal.pone.0190924

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© 2018 Knytl et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Metaphases of diploid CCAHe2Fi male (2n = 100), (A) Counterstained by DAPI showing consistently bright labelling of all 100 chromosomes with no positive blocks; B&W. (B) 5S rDNA probe showing 18 loci; green. Two strong signals are indicated by arrows. (C) Chromosomes stained by CMA₃ showing two NORs; B&W, (D) Pseudo-coloured with DAPI (blue), CMA₃ (green) indicated by arrows. (E) C-banded chromosomes showing 18 heterochromatic blocks located in the pericentromeric chromosome regions; B&W, (F) Pseudo-coloured with DAPI (blue). Scale bars = 10 μm.