Fig 2. Effects of WPCD on cytokine production and functional activation of DCs in vitro.

After DCs were treated with different concentrations of WPCD for 12 h, the supernatant was collected and the productions of IL-12 and TNF-α were detected by ELISA. The concentrations of IL-12 (A) and TNF-α (B) were shown. An allogenic T cell proliferation of WPCD-treated DCs was analyzed by MLR. Allogenic T cells were co-cultured for 3 days at the indicated ratio with DCs which had been cultured for 12 h in triplicate (C and D). MTT was added into the cultures for final 4-h treatment and the T cell proliferation was then determined by ELISA reader. Data represent mean ±SD (n = 3) from 3 independent experiments.*P < 0.05, ** P < 0.01, *** P < 0.001 compared with the untreated DCs group.