Fig 5. Effects of WPCD on the splenocyte proliferation.
Single splenocyte was isolated from mice immunized on day 21 after the first vaccination and splenocyte proliferation was determined by the MTT assay and expressed as SI. OVA-antigen stimulated splenocyte proliferation (A). OVA323-339-antigen stimulated splenocyte proliferation (B). ConA-mitogen stimulated splenocyte proliferation (C). LPS-mitogen splenocyte proliferation (D). Data are expressed as mean ± SD (n = 5) from 3 independent experiments. *P < 0.05, ** P < 0.01 compared with the OVA group; #P < 0.05, ##P < 0.01 compared with the OVA/Alum group.