Fig 6. Effects of WPCD on IL-4 and IFN-γ production from splenocytes.

On Day 21 after the first vaccination, single splenocyte was isolated from mice immunized and co-cultured with OVA (10 μg/mL) for 12 h. Productions of IFN-γ and IL-4 were determined by FCA_S. IL-4 production in CD4⁺ T cells (A), IFN-γ production in CD8⁺ T cells (B), and INF-γ production in CD8⁺ T cells (C). Data represent mean ±SD (n = 5) from 3 independent experiments. * P < 0.05, ** P < 0.01 compared with the OVA group; #P < 0.05, ##P < 0.01 compared with the OVA/Alum group.