Fig 7. Effects of WPCD on DCs maturation and Treg frequency.
On Day 3 or 21 after the first vaccination, cells were separated from the spleen of each group mice and the expression levels of CD11C+CD40+ and CD11C+CD80+ on DCs as well as the frequency of CD4+CD25+Foxp3+ was measured by FCAS. The expressions of CD11C+CD40+ (A) and CD11C+CD80+ (B) on DCs in the spleen on Day 3 after the first vaccination. The frequency of CD4+CD25+Foxp3+ Treg in the total CD4+ T cells in the spleen on Day 7 after the second vaccination (C). Data represent mean ± SD (n = 5) from 3 independent experiments. *P < 0.05 compared with the OVA group.