Table 1. Synergy between cdc73Δ and yku80Δ, tel1Δ, and exo1Δ in multiple GCR assays.
| Genotype | dGCR assay | sGCR assay | uGCR assay | |||
|---|---|---|---|---|---|---|
| RDKY | CanR 5FOAR Rate† | RDKY | CanR 5FOAR Rate† | RDKY | CanR 5FOAR Rate† | |
| Wild-type | 7635 | 8.1 [6.4–15] x 10−8 (1) | 7964 | 6.1 [4.3-18] x 10−9 (1) | 6677 | 2.27 [1.3–4.8] x 10−9 (1)* |
| cdc73Δ | 7986 | 7.54 [3.5–22] x 10−7 (9.3) | 8407 | 1.68 [1.1–3.0] x 10−7 (28) | 8480 | 1.56 [0.5–2.1] x 10−8 (6.9) |
| tel1Δ | 8340 | 3.38 [2.0–4.9] x 10−7 (4.2) | 8405 | 7.11 [5.8–8.5] x 10−9 (1.2) | 6761 | 4.99 [0.0–9.2] x 10−9 (2.2)* |
| yku80Δ | 8339 | 3.29 [1.5–10] x 10−7 (4.1) | 8406 | 3.25 [1.3–5.7] x 10−9 (0.5) | 8006 | <6.88 [0.0–7.9] x 10−10 (<0.3)* |
| cdc73Δ tel1Δ | 8324 | 1.91 [0.7–3.3] x 10−5 (236) | 8409 | 8.00 [3.9–11] x 10−7 (131) | 8481 | 2.14 [1.0–7.9] x 10−7 (94) |
| cdc73Δ yku80Δ | 8323 | 1.31 [0.7–3.4] x 10−5 (162) | 8411 | 1.51 [0.5–2.8] x 10−6 (248) | 8482 | 3.73 [2.2–5.2] x 10−7 (163) |
| tel1Δ yku80Δ | 8467 | 2.27 [1.3–3.0] x 10−6 (28) | 8408 | 1.86 [1.7–2.7] x 10−8 (3.1) | n.d. | |
| pif1Δ | n.d. | 8342 | 2.49 [1.6–3.5] x 10−6 (408) | n.d. | ||
| cdc73Δ pif1Δ | n.d. | 8343 | 4.36 [0.3–8.4] x 10−7 (72) | n.d. | ||
| exo1Δ | 8419 | 2.21 [1.6–2.7] x 10−7 (2.7) | 8469 | 1.09 [0.5–1.6] x 10−8 (1.8) | n.d. | |
| cdc73Δ exo1Δ | 8428 | 1.02 [0.8–3.5] x 10−5 (126) | 8470 | 2.22 [1.2–3.6] x 10−7 (36) | n.d. | |
| tel1Δ exo1Δ | 8464 | 6.13 [3.2–12] x 10−7 (7.6) | 8473 | 1.86 [0.6–3.0] x 10−8 (3.1) | n.d. | |
| yku80Δ exo1Δ | 8463 | 1.44 [1.2–2.2] x 10−7 (1.8) | 8472 | 8.78 [5.2–14] x 10−10 (0.14) | n.d. | |
* GCR rate from [51,52]. The uGCR rate determined using a wild-type uGCR strain constructed in RDKY7635, which is highly related to RDKY6677, was 1.8 [0.7–4.1] x 10−9.
†Rate of accumulating CanR 5FOAR progeny. The numbers in square brackets [] are the 95% confidence interval limits. The number in parenthesis () is the fold increase relative to the wild-type assay.
n.d., not determined.