Table 1.
Strains used in this study.
| Strain | Genotype or markers; characteristics and uses | Source or reference |
|---|---|---|
| Escherichia coli TOP10 | F–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| Thiobacillus denitrificans | ||
| ATCC 25259 | Wild type | American Type Culture Collection, Manassas, VA. |
| P2425 mutant | ATCC 25259 with Tbd_2545 gene replaced by PCR amplicon containing P2545-‘tesA-kana | This work |
| P2726 mutant | ATCC 25259 with Tbd_2545 gene replaced by PCR amplicon containing P2726-‘tesA-kana | This work |
| Pkan mutant | ATCC 25259 with Tbd_2545 gene replaced by PCR amplicon containing Pkan-‘tesA-kan | This work |
a
P2545 and P2726 represent promoters included in the upstream regulatory regions of Tbd_2545 (203 bp) and Tbd_2726 (218 bp), respectively.