Table 2.
PCR primers used in this study.
| Primer | Sequencea(5′−3′) |
|---|---|
| ltesA-f | ATGGCGGACACGTTATTG |
| ltesA-r-EcoRI | GGGGAATTCTTATGAGTCATGATTTAC |
| Ptbd_2545f-HindIII | GGGAAGCTTTTTCCGTCGGCGTACCGATC |
| Ptbd_2545r-tesA | ATCAATAACGTGTCCGCCATGATCTTCCCATCCATCCGAT |
| Ptbd_2726f-HindIII | GGGAAGCTTTGAGCGCGGGATCGAGAAGG |
| Ptbd_2726r-tesA | ATCAATAACGTGTCCGCCATTACAGTCTCCGTAGAGGTCG |
| kanP-f-HindIII | GGGAAGCTTAAAGCCACGTTGTGTCTC |
| tesA-f-kanP-r | CAGTAATACAAGGGGTGTTATGGCGGACACGTTATTG |
| Tbd_2545_KO1–1b | TACTCCTTGTCCAGCAGGTG |
| 2545UP-2-HindIII | GGGAAGCTTATCGCGGAACCTCTTTTCAT |
| KO3-EcoRIb | GCAGAATTCCAACCATCATCGATGAATTG |
| Tbd_2545_KO6–1b | TCGCGATCGCCTACATCGAC |
a
Relevant restriction sites are underlined.
b
Primer was previously used by Beller et al. (2013).