Table 1.

Description of identified selection pathways for enzymatic products xylitol, D-ribulose-1,5-bisphosphate, methanol, and aniline.

Desired product	Selection pathway label	Consumption pathway
For engineering Xylose Reductase
Xylitol	SPX1	xylitol+NAD+↔D-xylulose+NADH+H+
Xylitol	SPX2	xylitol+NAD(P)+↔L-xylulose+NAD(P)H+H+
		L-xylulose+NADH+H+↔L-arabitol+NAD+
		L-arabitol+NAD(P)+↔L-arabinose+NAD(P)H+H+
Xylitol	SPX3	xylitol+NAD(P)+↔L-xylulose+NAD(P)H+H+
		ATP+L-xylulose↔ADP+L-xylulose 5-phosphate
Xylitol	SPX4	xylitol+NAD(P)+↔L-xylulose+NAD(P)H+H+
		L-xylulose+NADH+H+↔L-arabitol+NAD+
		L-arabitol+NAD+↔L-ribulose+NADH+H+
Xylitol	SPX5	xylitol+NAD(P)+↔L-xylulose+NAD(P)H+H+
		L-xylulose↔L-lyxose
Xylitol	SPX6	xylitol+NAD(P)+↔L-xylulose+NAD(P)H+H+
		L-xylulose+CO2↔3-dehydro-L-gulonate
Xylitol	SPX7	xylitol+NAD(P)+↔L-xylulose+NAD(P)H+H+
		ATP+L-xylulose↔ADP+L-xylulose 1-phosphate
		L-xylulose 1-phosphate↔glycerone phosphate+glycolaldehyde
For engineering Phosphoribulokinase
D-ribulose-1,5-bisphosphate	SPD1	D-ribulose-1,5-bisphosphate↔D-ribose 1,5-bisphosphate
D-ribulose-1,5-bisphosphate	SPD2	D-ribulose-1,5-bisphosphate+CO2+H2O↔2 3-phospho-D-glycerate
D-ribulose-1,5-bisphosphate	SPD3	D-ribulose-1,5-bisphosphate+O2↔ 3-phospho-D-glycerate+2-phosphoglycolate
For engineering Methane Monooxygenase
Methanol	SPM1	methanol+NAD+↔formaldehyde +NADH+H+
Methanol	SPM2	methanol+formate↔2 formaldehyde+H2O
Methanol	SPM3	methanol+O2↔formaldehyde+H2O2
Methanol	SPM4	methanol+H2O2↔formaldehyde+2H2O
For engineering Aromatic Amino Acid Decarboxylase
Aniline	SPA	aniline+CO2↔4-aminobenzoate