Fig. 2.

Growth performance of the S. cerevisiae wild-type strain CBS 6412-13A (CBS) and engineered derivatives expressing a glycerol facilitator gene from C. jadinii (CBS CjFPS1), K. pastoris (CBS KpFPS1), P. tannophilus (CBS PtFPS2) or Y. lipolytica (CBS YlFPS1) in synthetic medium containing glycerol as the sole carbon source. The expression of all glycerol facilitators was controlled by the S. cerevisiae TEF1 promoter and the CYC1 terminator, and one copy of the respective expression cassette was integrated into the genome. The laboratory S. cerevisiae strain CEN.PK113-1A (CEN) not growing on glycerol at all was used as a negative control. The cells were grown in synthetic medium containing 6% (v/v) glycerol as the sole source of carbon and growth was recorded using the Growth Profiler 1152. For pre- and intermediate cultures synthetic medium with 2% (w/v) glucose was used. After glucose depletion the intermediate cultures were used to inoculate synthetic glycerol medium resulting in a lag phase due to the change of the carbon source. Maximum specific growth rates (µ_max) (A) and duration of lag phases after inoculation into synthetic glycerol medium (B) are shown. All mean values and standard deviations for error bar preparation were obtained from at least three biological replicates.