Table 1.
pH-controlled bioreactor experiments for characterizing the growth on glycerol of the S. cerevisiae strain CBS 6412-13A expressing FPS1 homologues from either C. jadinii DSM 2361 or P. tannophilus CBS 4044 in both wild-type and stl1 deletion background. All strains were cultivated in synthetic medium containing 10 g L−1 glycerol as the sole carbon source and pH was controlled at 5.0. Mean values and standard deviations for growth rates and yield coefficients (Ysx: biomass on substrate and Ysc: CO2 on substrate) were obtained from three biological replicates.
| Strain |
Growth rate (h−1) |
Yield coefficients (C-mol C-mol−1) |
||
|---|---|---|---|---|
| OD600 | Dry weight | Ysx | Ysc | |
| CBS 6412-13A CjFPS1 | 0.17±0.01 | 0.17±0.00 | 0.68±0.01 | 0.31±0.03 |
| CBS 6412-13A PtFPS2 | 0.15±0.01 | 0.15±0.01 | 0.69±0.01 | 0.30±0.00 |
| CBS 6412-13A CjFPS1 stl1Δ | 0.15±0.00 | 0.16±0.00 | 0.68±0.02 | 0.29±0.01 |
| CBS 6412-13A PtFPS2 stl1Δ | 0.15±0.01 | 0.16±0.00 | 0.68±0.02 | 0.29±0.01 |