Fig. 4.

PHBA production in pheromone treated cultures. A strain minimally engineered to produce PHBA (strain PHBA03) was grown with (purple lines, squares) and without (black lines, triangles) 1 µM alpha pheromone treatment at early exponential phase (OD of 1, at 4 h, indicated by vertical dashed lines). (a) Population density (OD_660 nm), (b) extracellular PHBA concentration, and (c) extracellular glucose concentrations were measured for shake-flask growth with and without pheromone over 48 h.(For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)