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Determination of plasmid copy number.
| Passages with selection | Passages without selection | ||||
|---|---|---|---|---|---|
| Plasmid | 1 | 5 | 1 | 2 | 5 |
| pDCW89 | 3.0±0.4 | 9.2±0.9 | 1.7±1.1 | 0.1±0.04 | 0 |
| pJGW37 | 6.7±0.6 | 7.5±1.7 | 0.9±0.1 | 0.1±0.05 | 0 |
Plasmid copy number was determined at mid-log phase after the indicated number of passages. Maintenance experiments were performed in biological duplicate. Uracil prototrophy was used to select for pDCW89. Resistance to thiamphenicol was used to select for pJGW37.
