Table 2.

Plasmid construction.

Constructed plasmid	Backbone source (restriction site)	Gene source (direct digestion or PCR)	PCR primers
pBbE2C-ORF27	pBbE2C-RFP (BglII, XhoI)	pDNA2.0-ORF27 (BglII, XhoI)	N/A
pBbS2C-ORF27	pBbS2C-RFP (BglII, XhoI)	pDNA2.0-ORF27 (BglII, XhoI)	N/A
pET28b-N-CaiC	pET28b (NdeI, XhoI)	pBbE2C-CaiC (NdeI, XhoI)	N/A
pBbE2C-ORF27	pBbE2C-RFP (BglII, XhoI)	pDNA2.0-ORF27 (BglII, XhoI)	N/A
pBbE2C-CaiC	pBbE2C-RFP (EcoRI, XhoI)	E. coli MG1655 gDNA PCR	JZ_CaiC_f, JZ_CaiC_r
pET28a-MBP-ORF27	pET28a-MBP (NdeI, XhoI)	pDNA2.0-ORF27 (NdeI, XhoI)	N/A
pBbE2C-MBP-ORF27	pBbE2C-RFP (BglII, XhoI)	pET28a-MBP-ORF27 PCR	JZ_MBP-ORF27_f, JZ_MBP-ORF27_r
pBbA5a-GadB	pBbA5a-RFP (NdeI, BamHI)	E. coli MG1655 gDNA PCR	JZ_GadB_f, JZ_GadB_r
pBbA5a-GadB_ΔHT	pBbA5a-RFP (NdeI, BamHI)	E. coli MG1655 gDNA PCR	JZ_GadB_f, JZ_GadB_ΔHT _r
pBbA5a-GadB_Δ1-14	pBbA5a-RFP (NdeI, BamHI)	E. coli MG1655 gDNA PCR	JZ_GadBΔ1-14_f, JZ_GadB_r
pBbA5a-GadB_Δ1-14, ΔHT	pBbA5a-RFP (NdeI, BamHI)	E. coli MG1655 gDNA PCR	JZ_GadBΔ1-14_f, JZ_GadB_ΔHT _r
pBbA7a-GadB	pBbA7a-RFP (NdeI, BamHI)	pBbA5a-GadB (NdeI, BamHI)	N/A
pBbA7a-GadB_ΔHT	pBbA7a-RFP (NdeI, BamHI)	pBbA5a-GadB_ΔHT(NdeI, BamHI)	N/A
pBbA7a-GadB_Δ1-14	pBbA7a-RFP (NdeI, BamHI)	pBbA5a-GadB_Δ1-14 (NdeI, BamHI)	N/A
pBbA7a-GadB_Δ1-14_ΔHT	pBbA7a-RFP (NdeI, BamHI)	pBbA5a-GadB_Δ1-14, ΔHT(NdeI, BamHI)	N/A