Fig. 4.

Individual reaction Knock-down and external metabolic co2 transport flux constraint effect on growth rate and EPS production: A) A graph describing the effect of constraining the hypothesized external metabolic co2 transport flux together with the individual reaction knock-down effect for all reactions on the predicted growth rate and EPS production is outlined. For each simulation, growth rate (specified in light blue bars in sub-graph on the left hand side y-axis) and EPS flux (specified in red bars in sub-graph on the right hand side y-axis) were plotted. In each sub-graph the corresponding biomass and EPS production rate was plotted for each one of the reactions (x-axis) in the model. Simulations were executed by adding an external co2 transport and constraining it in 0, 50% and 100% from the maximum capacity (x-axis), all reactions in the model were constraint between 0, 25, 50, 75% and 100% from their optimum capacities (y-axis). For each simulation the carbon uptake rate in the form of h2co3 was set to 2 mmol/g DW/h, oxygen and ferrous ion uptake rate were left unconstrained, and FBA was ran to maximize the flux through BOF. B) A set of graphs describing the production envelopes for reactions FUM and ASPK under the same conditions described previously. EPS (left y-axis in red lines) and co2 (right y-axis in green lines) were plotted as a function of growth rate (x-axis). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)