Fig. 5.
Relative RuBisCO content of engineered Synechocystis PCC 6803 strains control (WT+Kmr-vector), rbc, FL50, FL52, FL50strep, and FL52strep. A, SDS-PAGE and Western immunoblot, primary antibody anti-rbcL IgG was used to detect RuBisCO large subunit. B, RuBisCO content normalized to control strain determined by Quantity One. Each strain had biological duplicate. FL50 and FL52 had rbc gene, coding RuBisCO having FLAG tag fused to the large subunit N terminus or small subunit C terminus separately, overexpressed on pPMQAK1. In FL50strep and FL52strep, strep-tag II was used instead of FLAG tag. Standard derivation is from two biological replicates and two technical replicates. Asterisks indicate the differences observed between the respective engineered strain and the control strain are significant (One-way ANOVA, P<0.05). For strain information, see Table 1.