Table 1.
Synechocystis PCC 6803 strains used in this study.
| Strain | Expression construct | Expression site | Reference |
|---|---|---|---|
| WT+Kmr-vector | pPMQAK1 without ccdB | pPMQAK1 shuttle vector | (Liang and Lindblad, 2016) |
| rbc | PpsbA2-RBS*- slr0009-slr0011-slr0012 | pPMQAK1 shuttle vector | (Liang and Lindblad, 2016) |
| FL50 | PpsbA2-RBS*-FLAG- slr0009-slr0011-slr0012 | pPMQAK1 shuttle vector | This study |
| FL50C | PpsbA2-RBS*-slr0009-FLAG-slr0011-slr0012 | pPMQAK1 shuttle vector | This study |
| FL52 | PpsbA2-RBS*- slr0009-slr0011-slr0012-FLAG | pPMQAK1 shuttle vector | This study |
| FL50strep | PpsbA2-RBS*-strep II- slr0009-slr0011-slr0012 | pPMQAK1 shuttle vector | This study |
| FL52strep | PpsbA2-RBS*-slr0009-slr0011-slr0012-strep II | pPMQAK1 shuttle vector | This study |
| WT+Kmr-genome | Kanamycin cassette replaced slr0168 | Genome slr0168 | This study |
| FL50G | PpsbA2-RBS*-FLAG- slr0009-slr0011-slr0012 | Genome slr0168 | This study |
| FL52G | PpsbA2-RBS*- slr0009-slr0011-slr0012-FLAG | Genome slr0168 | This study |
| FL75 | A kanamycin cassette with PpsbA2 and FLAG tag inserted upstream of slr0009, after ATG of slr0009 | Upstream of slr0009 | This study |
| FL76 | FLAG tag and a kanamycin cassette inserted downstream of slr0012, in front of TAA of slr0012 | Downstream of slr0012 | This study |