Figure 5.

Inhibiting autophagy alleviates CISD2 silencing-induced glioma cell death. 3-MA was used to inhibit autophagy. Flow cytometry revealed that 3-MA significantly eliminated the si-CISD2-induced increase in (A) cell apoptosis and (B) necrosis. (C) Colony formation assays showed that 3-MA reversed the si-CISD2-induced decrease in mean colony number. (D) Wound-healing assays revealed that 3-MA abrogated the si-CISD-induced compromise in the invasive ability of U87 cells. Data are presented as the mean ± standard deviation from at least three independent experiments. *P<0.05 and **P<0.01, compared with Ctl group and si-CISD2+3-MA group; ^##P<0.01, compared with the indicated groups. CISD2, CDGSH iron sulfur domain 2; si-CISD2, small interfering RNA targeting CISD2; Ctl, control.