Figure 7.

CISD2 is a direct target of miR-449a. (A) Predicted binding site of miR-449a with CISD2 3′-UTR. (B) pGL3 vector containing CISD2 wild-type 3′-UTR or mutated form was co-transfected with or without miR-449a mimic into U87 cells. At 48 h post-transfection, the luciferase activity was measured. Data are presented as the mean ± standard deviation from at least three independent experiments. ^##P<0.01, compared with the indicated groups. CISD2, CDGSH iron sulfur domain 2; si-CISD2, small interfering RNA targeting CISD2; LC3-II, light chain 3 II; Atg7, autophagy related 7; 3′-UTR, 3′-untranslated region; miR, microRNA; wt, wild-type; mut, mutant.