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. 2017 Sep 28;16(6):7959–7966. doi: 10.3892/mmr.2017.7651

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Copyright: © Chou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Bufalin induced DNA fragmentation and damage in SCC-4 cells. Cells were treated with 0.5% DMSO, 1.25 µM H₂O₂, or 0, 100, 300 and 500 µM of bufalin for 48 h, and then DNA was isolated to analyze (A) DNA fragmentation (DNA ladder) by DNA gel electrophoresis or (B) to analyze DNA damage by a Comet assay and (C) quantitating the length of comet tails. Magnification, ×200. The results are presented as the mean ± standard deviation (n=3). **P<0.01 and ***P<0.001 vs. 0 nM bufalin (control). M, marker; C, control (0 nM bufalin); DMSO, dimethyl sulfoxide.