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. 2017 Sep 26;16(6):8019–8028. doi: 10.3892/mmr.2017.7616

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Comparison of osteogenic differentiation potential. (A) Alizarin red staining of unsorted cells, CD105⁺ SSCs, CD146⁺ SSCs and ASCs. Scar bar, 100 µm. (B) The mRNA levels of Runx2, ALP and OPN were detected using reverse transcription-quantitative polymerase chain reaction. All data are from 3 independent experiments and are presented as means ± SD. *P<0.05 vs. unsorted cells; ^#P<0.05 vs. ASCs, ^αP<0.05 vs. CD105⁺ SSCs. RUNX2, runt-related transcription factor 2; ALP, alkaline phosphatase; OPN, osteopontin; ASCs, adipose-derived stem cells; SSCs, skeletal stem cells; CD, cluster of differentiation.