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. 2017 Sep 22;16(6):8062–8068. doi: 10.3892/mmr.2017.7587

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Copyright: © Hui et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Replacement of 2% HS with 10% FBS ahead of PAS staining detection reflects the glycogen storage and accumulation function of induced HP14.5d cells. HP14.5d cells were treated with DMEM containing 2% HS + 0.1 µmol/l Dex + 10 ng/ml HGF + 20 ng/ml FGF4 for 12 days, then the induction medium was replaced with complete DMEM with 10% FBS + 0.1 µmol/l Dex + 10 ng/ml HGF + 20 ng/ml FGF4 for different time periods. (A) PAS staining and (B) ICG uptake were assessed at 12, 24, 48 and 72 h after the induction medium was changed. (a) Uninduced HP14.5d cells as control; (b) HP14.5d cells induced for 12 days with no media shift; (c) HP14.5d cells induced for 12 days and induction medium changed for 12 h; (d) HP14.5d cells induced for 12 days and induction medium changed for 24 h; (e) HP14.5d cells induced for 12 days and induction medium changed for 48 h; (f) HP14.5d cells induced for 12 days and induction medium changed for 72 h. Photomicrographs were captured under ×100 magnification. Representative images and data from ≥3 independent experiments are presented. *P<0.05 vs. control; ^#P<0.05 among different induced groups. HS, horse serum; FBS, fetal bovine serum; PAS, periodic acid-Schiff; DMEM, Dulbecco's modified Eagle's medium; Dex, dexamethasone; HGF, hepatic growth factor; FGF4, fibroblast growth factor 4; ICG, indocyanine green.