Figure 4.

Knockdown of E2F1 inhibits proliferation and induces apoptosis in MCF-7 cells. Relative miR-372 (A) mRNA and (B) protein expression levels in MCF-7 cells transfected with si-E2F1 or si-NC, as detected by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. (C) Proliferation of transfected MCF-7 cells measured by MTT assay. (D) Apoptosis of transfected MCF-7 cells, as analyzed by flow cytometry. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05. si, small interfering; miR-372, microRNA-372; NC, negative control; OD, optical density; E2F1, transcription factor E2F1.