Figure 4.

The Rho-ROCK2 pathway mediates simvastatin-inhibited CyPA secretion. (A) Growth-arrested VSMCs were pretreated with GGOL for 30 min, and simvastatin was added for 30 min prior to exposure to 1 µmol/l LY83583 for 2 h. (B) Growth-arrested VSMCs were pretreated with KD025 and GGTI-298 for 30 min prior to exposure to 1 µmol/l LY83583 for 2 h. CyPA in the CM and TCL were detected by western blotting. All the experiments were repeated at least three times. GGOL, geranylgeraniol; Sim, simvastatin; CM, conditioned medium; TCL, total cell lysate; CyPA, cyclophilin A; GGTI, geranylgeranyltransferase inhibitor; ROCK2, Rho-associated protein kinase 2; VSMCs, vascular smooth muscle cells.