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. 2017 Sep 26;16(6):8155–8163. doi: 10.3892/mmr.2017.7636

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — TGF-β regulates mouse airway progenitor cells through its receptors on MLg cells. (A) Exclusion of 7AAD^pos cells (y-axis). (B) CD34/31/45(Lin) (x-axis) vs. EpCAM (y-axis) analysis of 7AAD^neg population from A. (C) Sca-1 (x-axis) vs. GFP (y-axis) analysis of Lin^neg/EpCAM^pos population from B represents airway progenitor cells. (D) Sorted progenitor cells were cultured in basic medium, with an addition of 10 µM SB431542, 10 µM SB431542 and MLg cells at day 6. (E) Sorted progenitor cells from Sftpc-Cre⁻; TGF-β2R^f/f and Sftpc-Cre⁺; TGF-β2R^f/f mice were cultured under control conditions and with an addition of 10 µM SB431542 for 4 weeks. (F) Quantitative analysis of the colony-forming efficiency in different culture conditions. Data are expressed as the mean ± standard error of the mean. *P<0.05 as indicated. Scale bars, 200 µm. TGF-β, transforming growth factor-β; FSC, forward scatter; GFP, green fluorescent protein; 7-AAD, 7-amino-actinomycin D.