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. 2017 Oct 4;16(6):8685–8690. doi: 10.3892/mmr.2017.7708

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Copyright: © Lin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — WWC1 impacts dendrin nucleus relocation in podocyte. (A and B) Podocytes were transfected with WWC1 siRNA or scramble siRNA. Podocytes with either WWC1 or scramble siRNA transfection were lysated and nuclear protein was extracted using the nuclear and cytoplasmic protein extraction kit. Nucleus dendrin expression was measured by western blotting, using histone 3 as equivalent loads in the nuclei. (C) Representative photographs of triple staining of DAPI, WWC1 and dendrin showing nucleus relocation of dendrin in podocytes with silenced WWC1 gene (original magnification, ×400). Analysis of variance, *P<0.05, vs. Scr. siRNA, small interfering RNA; Scr, scramble siRNA.