Figure 4.

Metformin therapy enhances wound angiogenesis in db/db mice. A dorsal skin wound was created via a 6-mm circular punch biopsy and wound angiogenesis was measured on days 7 and 14. (A) Representative images of CD31 staining on day 7 and (B) quantitative analysis. (C) Representative images of CD31 staining on day 14 and (D) quantitative analysis. Red arrows indicate CD31-positive capillaries (magnification, ×200; scale bar, 50 µm). Quantitative analysis of capillaries in each field demonstrated that wound capillaries in metformin-treated db/db mice were increased on days 7 and 14 when compared with the untreated db/db mice. ***P<0.001, **P<0.01 vs. Con; ^##P<0.01, ^#P<0.05 vs. db/db. Values are expressed as the mean ± standard error of the mean (n=5 mice/group). Con, control; Met, metformin; hpf, high-power field.