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. 2017 Oct 4;16(6):8747–8754. doi: 10.3892/mmr.2017.7721

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — High expression of MACC1 in 5-FUR colon cancer cells and RNA interference-mediated MACC1 knockdown. (A) Western blot analysis of MACC1 expression in SW620 and HCT116 parental cells and 5-FUR cells. (B) SW620 and HCT116 cells transduced with the LV-MACC1-shRNA-GFP visible in normal light and under a fluorescent microscope. Merged images are presented (magnification, ×200). (C) Bar graphs depict relative expression levels of MACC1 protein and (D) mRNA. LV-MACC1-shRNA exerted inhibitory effects on MACC1 expression, as identified by reverse transcription-quantitative polymerase chain reaction and western blot analysis. *P<0.05, **P<0.01. 5-FUR, 5-fluouracil-resistant; GFP, green fluorescent protein; LV, lentivirus; MACC1, metastasis-associated colon cancer 1; ns, not significant; shRNA, short hairpin RNA.