Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 4;16(6):8747–8754. doi: 10.3892/mmr.2017.7721

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — MACC1 knockdown reduces cancer stem cell-like properties of 5FUR colon cancer cells. (A) Sphere forming ability was evaluated in the vehicle group cells and shRNA group cells (magnification, ×200). Bar graphs demonstrate the relative sphere numbers. (B) Protein levels of CD44, Nanog and CD133 were analyzed by western blotting in the vehicle group and shRNA group cells. Bar graphs demonstrate the relative protein expression levels. (C) mRNA levels of the aforementioned genes were analyzed by reverse transcription-quantitative polymerase chain reaction in the vehicle group and shRNA group cells. Bar graphs demonstrate the relative mRNA expression levels. *P<0.05, **P<0.01. 5-FUR, 5-fluouracil-resistant; CD, cluster of differentiation; MACC1, metastasis-associated colon cancer 1; Oct-4, octamer-binding transcription factor 4; shRNA, short hairpin RNA.