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. 2017 Oct 3;16(6):8826–8832. doi: 10.3892/mmr.2017.7701

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Copyright: © Cheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of HTY and PEMF on cell apoptosis under H2O2 stimulation in human umbilical vein endothelial cells. (A) Representative fluorescence images and (B) quantification of TUNEL-positive cells. DAPI staining represents the nucleus. (C) Representative fluorescence images and (D) quantification of the ratio of apoptotic cells at 24 h, as assessed by calcein acetoxymethyl/PI staining. Data are expressed as the mean ± standard error of six independent experiments. ^##P<0.01 vs. PEMF-treated group. Magnification, ×200. HTY, hydroxytyrosol; PEMFs, pulse electromagnetic fields; Ctrl, control; PI, propidium iodide; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling.