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. 2017 Oct 9;16(6):8907–8915. doi: 10.3892/mmr.2017.7744

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Overexpression of ID1 promotes the proliferation of SACC-83 cells. (A) Western blotting analysis of ID1 protein expression levels in SACC-83 cells transfected with a recombinant plasmid (pEX-2) containing the ID1 sequence or empty vector controls. Following plasmid transfection, the proliferation of SACC-83 cells was detected using the (B) Cell Counting kit-8 assay at 0–96 h (**P<0.01 vs. vector-only transfected cells), and a (C) colony formation assay (**P<0.01 as indicated). The results are presented as the mean ± standard deviation (n=3). ID1, inhibitor of DNA binding 1; SACC, salivary adenoid cystic carcinoma; OD, optical density.