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. 2017 Oct 9;16(6):8907–8915. doi: 10.3892/mmr.2017.7744

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Knockdown of ID1 inhibits the migration and invasion of SACC-83 cells. (A) Representative images of SACC-83 cell migration after transfection with siRNA800, siRNA858 or NC at 0 and 24 h following generation of the scratch-wound (magnification, 40x). (B) Representative images of the Transwell migration (upper panels) and invasion (lower panels) assays following transfection of SACC-83 cells with ID1 siRNAs or NCs (magnification, ×200). (C) The number of cells that migrated through the uncoated filters (no Matrigel), represented the migratory ability of the SACC-83 cells. (D) The number of cells that passed through filters pre-coated with Matrigel represented the invasive ability of SACC-83 cells. The cell counts are presented as the mean values/field of view from at least five randomly selected low-powered fields from three independent experiments. The results are presented as the mean ± standard deviation. **P<0.01 as indicated. ID1, inhibitor of DNA binding 1; SACC, salivary adenoid cystic carcinoma; siRNA, small interfering RNA; NC, negative control.