Table I.
Gene | Primer sequence |
---|---|
SOX-9 | Forward 5′-CATGAACGCCTTCATGGTGT-3′ |
Reverse 5′-CCACACCTCCTCTTCTTTCT-3′ | |
AGN | Forward 5′-ATCCCAGAAAACTTCTTTGG-3′ |
Reverse 5′-TTCTACTCACTCCCCCTT-3′ | |
Col II | Forward 5′-AACAGCCAAAGGACCCAAGT-3′ |
Reverse 5′-AAATAACACAACCACCCTCT-3′ | |
Col X | Forward 5′-TTCCATTTGATAAGATTTTG-3′ |
Reverse 5′-ACCTACACTCTCTTTTACTC-3′ | |
HPGD | Forward 5′-CGGGCATGAGTCCTGCTAAA-3′ |
Reverse 5′-TCACCCCTTCCCTTTCTACC-3′ | |
Col I | Forward 5′-TCTTTTTCCTCTTCTTCTTT-3′ |
Reverse 5′-TTTTTATTTTATTTTTCCTA-3′ | |
β actin | Forward 5′-CCAACCGCGAGAAGATGA-3′ |
Reverse 5′-CCAGAGGCGTACAGGGATAG-3′ |
SOX-9, transcription factor Sox9; AGN, aggrecan; Col, collagen; HPGD, 15-hydroxyprostaglandin dehydrogenase [NAD(+)].