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. 2017 Oct 12;16(6):9375–9382. doi: 10.3892/mmr.2017.7784

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Copyright: © Xiao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Inducing apoptosis via the combination of CD55-TRAIL and luteolin. (A) After 48 h, nuclear fragmentation (arrows) was observed in HT-29 (a-d) and Beas-2B (e-h) cells using Hoechst staining under an inverted fluorescence microscope (0.2 mm fields; magnification, ×200). The a and e were untreated. The b and f were treated with luteolin. The c and g were treated with CD55-TRAIL. The d and h were treated with the combination of CD55-TRAIL with luteolin. (B) The percentage of apoptotic cells was detected using flow cytometric analysis. The data are presented as the mean ± standard deviation. n=3. *P<0.05. (C) Apoptosis-associated protein expression was detected by western blotting. GAPDH was used as the internal control. CD55, complement decay-accelerating factor; TRAIL, tumor necrosis factor ligand superfamily member 10; XIAP, E3 ubiquitin-protein ligase XIAP; PARP; poly-ADP-ribose polymerase.