Table I.
Gene | Primer Sequence (5′-3′) | Length (bp) |
---|---|---|
ALP | F: ACACTCGGCCGATCGGGACT | 20 |
R: CCGCCACCCATGATCACGTCG | 21 | |
OCN | F: TAGTGAACAGACTCCGGCGCTA | 22 |
R: TGTAGGCGGTCTTCAAGCCAT | 21 | |
OPG | F: GTGGAATAGATGTCACCCTGTGT | 23 |
R: TTTGGTCCCAGGCAAACTGT | 20 | |
RANKL | F: CAGAAGATGGCACTCACTGCA | 21 |
R: CACCATCGCTTTCTCTGCTCT | 21 | |
GAPDH | F:AGCAGTCCCGTACACTGGCAAAC | 23 |
R:TCTGTGGTGATGTAAATGTCCTCT | 24 |
F, forward; R, reverse; ALP, alkaline phosphatase; OCN, osteocalcin; OPG, osteoprotegerin; RANKL, tumor necrosis factor ligand superfamily member-11.