Figure 6.

Western blot analyses. (A) Effects of DOX on the expression of cleaved caspase-3 after 6–12 h of H9c2 cell exposure to DOX at 5 µM. (B) Effects of NRG and p38MAPK inhibitor on DOX-induced increase in cleaved caspase-3 expression. The cells were treated for 12 h by using 5 µmol/l DOX in the presence or absence of 150 min of pretreatment by 1 µM NRG or 60 min of pretreatment by 3 µM SB203580. The data were quantified by ImageJ software using densitometric analysis and expressed as mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 vs. Control; ^##P<0.01, ^#P<0.05 vs. DOX-treated group, NRG, naringin; DOX, doxorubicin.