Figure 7.

Immunofluorescence staining of reactive oxygen species in differently treated H9c2 cells. (A) Control group. (B) The cells were exposed for 24 h to DOX at 5 µM. (C) The cells were pretreated for 150 min by NAG at 1 µM before 24 h of exposure to DOX at 5 µM. (D) The cells were pretreated for 60 min by 3 µM SB203580 before 24 h of exposure to DOX at 5 µM. (E) The cells were treated for 150 min by 1 µM NRG and cultured for 24 h. (F) The cells were treated for 60 min by 3 µM SB203580 and cultured for 24 h. (G) Quantified MFI of DCF in A-F by Image J software. The data are expressed as mean ± standard error of the mean (n=3). Magnification, ×400. *P<0.05 vs. Control; ^#P<0.05 vs. DOX-treated group. DOX, doxorubicin; NAC, N-acetyl-L-cysteine; NRG, naringin; DCF, dichlorofluorescein; MFI, mean fluorescence intensity.