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. 2017 Oct 13;16(6):9521–9527. doi: 10.3892/mmr.2017.7789

Figure 2.

Figure 2.

Evaluation of the caveolin-1 silencing mediated by lentiviral short hairpin RNA. (A) The transduction efficiency was ~90%, as determined by fluorescence microscopy (magnification, ×40; scale bars, 500 µm). (B) Assessment of caveolin-1 mRNA expression by reverse transcription-quantitative polymerase chain reaction at 24, 48 and 72 h following t-BHP-induced oxidative stress. In the NP-LV group caveolin-1 mRNA levels were reduced to ~46% of those produced by the NP-CTR group at 72 h. *P<0.05, as indicated. (C) Protein levels were assessed by western blotting. The expression of caveolin-1 was markedly decreased in the NP-LV group at 72 h when compared with the other two groups. t-BHP, tert-butyl hydroperoxide; NP, nucleus pulposus; NP-CTR group, control cells treated with t-BHP only; NP-LV group, cells treated with t-BHP and lentivirus; NP-LV-NC, cells treated with t-BHP and negative control lentivirus.