Figure 6.

α7nAChR expressed in THP-1 derived macrophages (TMs) inhibits the migration of CRC cells through the JAK2/STAT3 signaling pathway. TMs were treated with or without α-bungarotoxin (α-Btx) for 6 h and then with DMSO (control), AG490, LY294002 and Bay 11–7082 for 1 h, respectively. Subsequently, LoVo cells were co-cultured with TM in 24-well Transwell plates with 8 µm polycarbonate membrane filters for 36 h for the migration assay. (A) Upper panel: Representative images show the migration of LoVo cells under the condition that the TMs did not have the α-Btx treatment and were treated with AG490, LY294002 and Bay 11–7082 only, respectively. Lower panel: Representative images show the migration of LoVo cells under the condition that the TMs were treated with α-Btx only, or dually treated with AG490, LY294002 and Bay 11–7082, respectively. (B) Number of migrated LoVo cells are presented as the mean ± SD of six images (n=6) of three separate experiments. Significance of differences between groups was determined by Student's t-test. P-value <0.05 indicates that there was a significant difference between treatments.