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. 2017 Sep 22;38(5):2705–2716. doi: 10.3892/or.2017.5989

Figure 3.

Figure 3.

PF-3758309 induces cell cycle arrest and apoptosis in neuroblastoma cells. (A) Clone formation assay of SH-SY5Y and IMR-32 cells incubated with DMSO or PF-3758309 for 2 weeks. (B) Clone numbers of SH-SY5Y and IMR-32 cells incubated with DMSO or PF-3758309 for 2 weeks. (C) PI staining analysis showed neuroblastoma cells underwent cell cycle disorder upon exposure to PF-3758309. SH-SY5Y cells were harvested after 24 h of treatment with 1 or 5 µM PF-3758309. IMR-32 cells were treated for 24 h with PF-3758309 at 0.5 or 1 µM compared with DMSO control mock treatment. (D) Proportion of cells in the G1/S/G2 phase after 1 or 5 µM PF-3758309 treatment in SH-SY5Y cells and 0.5 or 1 µM PF-3758309 treatment in IMR-32 cells. (E) Annexin V/PI staining of NB cells incubated with PF-3758309 for 24 h. SH-SY5Y cells were harvested after 24 h of treatment with 1 or 5 µM PF-3758309. IMR-32 cells were treated for 24 h with PF-3758309 at 0.5 or 1 µM compared with DMSO control mock treatment. (F) Proportion of apoptotic cells after 1 or 5 µM PF-3758309 treatment in SH-SY5Y cells and 0.5 or 1 µM PF-3758309 treatment in IMR-32 cells; **P<0.01 and ***P<0.001. P-values were determined by two-tailed t-tests. All data are representative of 3 independent experiments with n=3–6/group and are means ± SEM.