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. 2017 Sep 22;38(5):2705–2716. doi: 10.3892/or.2017.5989

Figure 5.

Figure 5.

PF-3758309 induces cell death through the ERK signaling pathway. (A) Western blot analysis of phosphorylation of ERK, Akt and p38 in lysates from SH-SY5Y and IMR-32 cells treated with serial concentrations of PF-3758309 for 24 h. The phosphorylation of ERK decreased upon PF-3758309 treatment in both cell lines, but no obvious change in the phosphorylation of Akt was observed. The phosphorylation of p38 was increased in the IMR-32 cells treated with PF-3758309, but no obvious change was noted in SH-SY5Y cells. (B) Western blot analysis of the phosphorylation of ERK, Akt and p38 in lysates from SH-SY5Y and IMR-32 cells treated with 1 µM PF-3758309 at different time points. The phosphorylation of ERK decreased upon PF-3758309 treatment over time in both cell lines, but no obvious change in the phosphorylation of Akt was observed. The phosphorylation of p38 was increased in IMR-32 cells treated with PF-3758309, but no obvious change was noted in the SH-SY5Y cells. All data are representative of 3 independent experiments with n=3–6/group and are means ± SEM.